Lycium barbarum polysaccharides (LBPs) from goji berries extended mean lifespan by 20.67% in C. elegans worms at 700 μg/mL concentration, while enhancing stress resistance, locomotion, and reducing age-related cellular damage markers. The longevity effects required activation of three key transcription factors: DAF-16/FOXO, SKN-1/Nrf2, and HSF-1, operating through the insulin/IGF-1 signaling pathway and lipid metabolism genes including fat desaturases. This mechanistic validation in a well-established longevity model organism provides compelling evidence for goji berries' traditional reputation as a longevity food. The insulin pathway involvement is particularly significant since this represents one of the most conserved aging mechanisms from worms to humans. However, translating these dramatic worm lifespan extensions to human benefits remains highly speculative. The 700 μg/mL concentration used exceeds typical dietary intake levels, raising questions about practical dosing. While C. elegans studies have predicted several successful human longevity interventions, the leap from 20-day worm lifespans to human longevity requires substantial additional research. This represents solid confirmatory evidence for goji polysaccharides' bioactivity rather than paradigm-shifting discovery.